Alterations in Semen Quality by Antioxidants in Stressed Healthy Individuals


  • Saurabh Saha1 , Sonali Saxena2 , R.K. Srivastava3 , Chitra Srivastava4



Antioxidants, stress, Sperm count, Sperm Motility, Sperm morphology.


The study was conducted to evaluate the role of antioxidants in current therapeutic use on stress induced by

exercise on sperm profile. 68 unmarried healthy medical students between 18-24 years of G.S.V.M Medical

College who with informed written consentacted as volunteers for study duration of 90 days. Their identities

were strictly confidential and were selected randomly. Complete and fresh specimen semen was collected

by accepted method of masturbation after abstinence for more than 3 days. Two samples were taken first on

commencement and second on conclusion of 3 monthperiod. The subjects have been divided into 4 groups

of 17 subjects in a single blinded study. Group I- control and were administered placebo capsules containing

arrow root powder. Group II-administered placebo capsules and performed moderate exercise daily for 30

minutes for 3 months on a bicycle ergo meter with persistent increase in heart rate 30 to 50 per minute above

their basal heart rate. The subjects carried out exercised for 30 minutes or exercise was stopped due to pain.

Group III- carried out the exercise with the same protocol as in group II and were given 1 capsule daily of

antioxidant preparation, ECarotin (Franco Indian Pharmaceuticals, India) composition Beta carotene 10 mg,

vitamin E acetate I.P. 25 mg, Vitamin C I.P.150 mg, Selenium dioxide monohydrates U.S.P. 75 mcg, Zinc

sulphate monohydrate U.S.P. 61.8 mg (equivalent to elemental zinc 22.5 mg). Group IV - Subjects of this

group did not perform any exercise and were administered 1 capsule daily of ECarotin. The following physical

features of the ejaculate were recorded Color, Liquefaction Time, pH, Volume Microscopic Examination

included Sperm Count, Motility, Sperm morphology while Biochemical estimation done for seminal fluid

Fructose. A statistically significant (p < .005)decrease in sperm countin group II (exercising and taking

placebo) and group III (exercising and taking [ECarotin]) but group IV taking only (ECarotin) showed a

significant increase in sperm count (p < 0.05).Group’s III and IV consuming antioxidant (ECarotin) showed

a significant increase in the number of motile sperms (p < 0.05) and a significant decrease in the number of

non-motile/dead sperms (p < 0.05). Most of the subjects had pus cell in mild to moderate quantity.

Author Biography

Saurabh Saha1 , Sonali Saxena2 , R.K. Srivastava3 , Chitra Srivastava4

1Associate Professor (Physiology), 2Associate Professor (Medicine), 3Professor (Anatomy), 4Associate Professor (Physiology), Department of Physiology, G.S.V.M Medical College, Kanpur



How to Cite

Saurabh Saha1 , Sonali Saxena2 , R.K. Srivastava3 , Chitra Srivastava4. (2020). Alterations in Semen Quality by Antioxidants in Stressed Healthy Individuals. International Journal of Physiology, 8(3), 86-90.