Evaluation of TNFα in Patients with Heart Failure According to BNP Concentration
Background: Heart failure (HF) is a condition in which the heart can no longer pump blood as efficiently
as it used to. This is lead to a complex of clinical symptoms like (dyspnea, orthopnea, lower limb swelling).
BNP is a neurohormone released from myocardium and increased when failure of heart is occure and used
for diagnosing heart failure. TNFα is pro inflammatory cytokine, its concentration increasing in patients with
heart failure, this caused by inflammation which is present in heart with failure.
Objectives: This study was designed for Examining serum concentrations of TNFα in patients with HF,
examining whether this cytokines was involved in the pathophysiology of the HF syndrome, and investigating
the effectiveness of using BNP concentration as an indicator for heart failure.
Subjects and Method: The current study is a cross sectional study that was carried out in the center of
Diyala province, Baquba Teaching Hospital, to determine the serum concentration of BNP and TNF-α in
patients with heart failure. The samples were collected in 2 and a half months, from January the 15th to
Aprilthe 1st 2019.
A total of 150 serum samples were collected . 100 patients with diagnosed heart failure, and the rest
apparently healthy. The age range was 30-89 years, 50 of them were males, and 50 were females. BNP and
TNFα concentrations were tested in the serum samples using the BNP and TNFα ELISA kit (from Sun Red
company - China).Human privacy was respected by taking the parents’ verbal consent. Statistical analysis
of data was carried out using the Statistical Packages for Social Sciences (SPSS), Version 25. Statistical
significance was considered whenever the P value was equal to or less than 0.05.
Results: The results showed that the BNP concentration range in HF group patients (50-1600) was wider
than that in healthy control group (5-160) with a statistically significant difference between HF group
and healthy control group(P = 0.0001).Also there is statistically significant association between TNFα
concentration and HF (P = 0.005). Furthermore, there was no statistically significant effect of age and
gender on TNF-α concentration in neither the diseased group nor the control group. Also there is statistically
significant relationship between TNFα and family history (P = 0.026). There is direct relationship between
TNFα and HF grade with a P value of 0.0001.
Conclusion: The concentration of TNFα is elevated with increasing the severity of heart failure accompanied
by BNP concentration elevation, where Theincrease the HF grade, the elevation the concentration of TNFα.