Comparison and Correlation of CD4T Cells Countwith Viral Load Prior to and after Initiating HAART in HIV Iraqi Patients
Background: HIV that cause of AIDS worldwide distributed and the CD4 positive T cells consider chief
target cells of HIV. Elimination of HIV is the final goal of HIV treatment, but is rarely achieved.
Objective: As CD4T cells count using flow cytometry recently available, we investigated whether CD4T
cells count can substitute for HIV plasma viral load RNA quantification in treatment monitoring through
comparison the change in CD4 positive T cells count in patients with HIV previous to & afterward initiating
״highly/active/antiviral/therapy ״ʻHAARTʼ & correlation of CD4 T cellscounts with viral load prior to and
after initiating HAART in HIV Iraqi patients.
Method: Within this study, 25 HIV patients were prospectively analyzed. Patients have been treated with
Bilateral/Didanosine, combination (Efavirenz, Emtricitabine, and Tenofovir Disoproxil Fumarate), in
addition to Quadruple combination (Elvitegravir,cobicistat,emtricitabine,tenofovir alafenamide (TAF).
Quantitative CD4T cells countwere determined with flowcytometry. HIV plasma viral load RNA were
determined with RT-PCR at given time points.
Results: We find weak negativenon-significantʺ correlationʺ(R≈-0.267, p≈ 0. 197 & R≈-0.161, p≈0. 441)
among CD4positive T cellscounts & ʾplasma viral load of HIV RNAʾin both treated and untreated patients
respectively. More importantly, there was significant concordance between an increase or decrease of CD4T
cells counts with HIV RNA plasma viral load prior and after initiating of treatment. However, the curve and
increase of CD4T cells count enabled prediction of eventual of viral clearance.
Conclusions: Quantitative CD4T cells count cannot substitute for HIV RNA plasma viral load quantification
during assessment of antiviral therapy: However, the increase of CD4 T cells count does predict eventual
HIV RNA plasma clearance. A 2 log 10 increase to above 200 IU/ml is associated with a high likelihood of
HIV plasma RNA clearance.