Phytochemical Analysis, Antioxidant and Cytotoxic Potential of Rumex Vesicarius Extracts

  • Student Sabreen A. Alazzam1, Mohammed Mosleh Sharqi2, Ali F. AlMehemdi3
Keywords: Rumex vesicarius, Medicinal plants, Cytotoxicity, GC-MS analysis, High content screening.

Abstract

Background: Rumex vesicarius L. (Polygonaceae), an edible plant, is documented to have many bioactive
phytochemicals. In spite of it fully -known antioxidant efficacy of crude extract Rumex vesicarius but still
has the cytotoxicity.
Methodology: Methanolic extraction was prepared from leaves of Rumex vesicarius and was checked by
GC/MS. Two parts of assay were classified as : First, evaluation antioxidant potency using DPPH free
radicals, while the two assay of MTT was utilized to determine anti-cancer effect on two different cell lines
(MCF-7 and WRL68) for different concentrations (400μg/ml, 200μg/ml, 100μg/ml, 50μg/ml and 25μg/
ml and 6.2 μg/mL) and identified the efficacy of the crude extract on MCF-7 cell morphology, response to
stress, potential of mitochondria and viability were evaluated with High Content Scanning and MTT.
Results: GC/MS analysis showed the methanol extract of Rumex Vesicarius L. had the highest percentage
of L- Pidolic Acid (35.98%), vitamin E (12.05%). R. Vesicarius showed antioxidant activity which increased
when increasing of concentration, R. Vesicarius effect ranged from (75.935,9.96%). The results showed
Rumex vesicarius ability to inhibit cellular growth of both cell lines (WRL68 and MCF-7). This inhibition
increases with increasing concentration. The best inhibition of growth was at 400μg/ml of Methanol extract.
The MCF-7 cell line was more effective than the WRL68 cell line, High Content Screening (HCS) assay of
200μg/ml, 100μg/ml, 50μg/ml, 25μg/ml and 12.5μg/ml of methanol extract of R. Vesicarius showed toxic
effects toward MCF-7 cell line after 24 hours of treatment in a dose-dependent manner with a reduction in
the number of total cell count, reduction in the mitochondrial membrane potential (MMP), an increase in the
nuclear intensity, increase in the membrane potential and increase in cytochrome C.
Conclusion: Methanolic extract of Rumex vesicarius contains many bioactive phytochemicals and appear
to have cytotoxic and antioxidant activity against MCF-7 & WRL68 Cell line.

Author Biography

Student Sabreen A. Alazzam1, Mohammed Mosleh Sharqi2, Ali F. AlMehemdi3

1Department of Biology, College of Science, 2Lecture, 3Prof., Center of Desert Studies, University of Anbar, Iraq

Published
2020-05-22
How to Cite
Student Sabreen A. Alazzam1, Mohammed Mosleh Sharqi2, Ali F. AlMehemdi3. (2020). Phytochemical Analysis, Antioxidant and Cytotoxic Potential of Rumex Vesicarius Extracts. Medico Legal Update, 20(2), 449-454. Retrieved from http://ijop.net/index.php/mlu/article/view/1146