Evaluation of the Role of miRNA-21 Levels as a Potential Diagnostic Biomarker for Colorectal Cancer Associated with Prognosis

  • Ekhlas Saddam Falih1, Suhad Hassan Obaid1, Faten Rashid Hameed2
Keywords: Colorectal cancer, MiRNA-21, biomarker

Abstract

Colorectal cancer one of the most common and aggressive tumor diagnosed in humans. The potential role
has been suggested that circulating microRNAs assuring diagnostic markers for early detection of colorectal
cancer .Hereabouts, this investigation intends to study the performance of miRNA-21 in CRC. Fifty blood
samples were collected from individuals with colon cancer who enrolled in the Teaching Laboratories of
the Medical City/Baghdad from June 2018 to September 2019. Taq Man microRNA Real-time PCR was
utilized to recognize the expression of miRNA-21 in the patient’s plasma. Representative in the expression
of miRNA-21 by gender and pathological grading were not statistically important (P>0.05). Moreover,
alterations in the expression of miRNA-21 amongst patients and control were significances statistically
(P<0.05). While a higher risk of the disease was associated with high miRNA-21 and correlated with poor
prognosis. The AUC for miRNA-21 was 0.657. The optimal cut-off value was 1.961-fold with sensitivity
and specificity 64%, 68% respectively. In Conclusion, the high miRNA-21 expression might be a prognostic
marker of colon cancer patient consequently; high expression of miRNA-21 was associated with poor
prognosis of patients with colorectal cancer.

Author Biography

Ekhlas Saddam Falih1, Suhad Hassan Obaid1, Faten Rashid Hameed2

1Lecturer, 2Assist. Prof., Middle Technical University, Collage of Health & Medical Technology, Baghdad

Published
2020-05-22
How to Cite
Ekhlas Saddam Falih1, Suhad Hassan Obaid1, Faten Rashid Hameed2. (2020). Evaluation of the Role of miRNA-21 Levels as a Potential Diagnostic Biomarker for Colorectal Cancer Associated with Prognosis. Medico Legal Update, 20(2), 508-513. Retrieved from http://ijop.net/index.php/mlu/article/view/1158