Evaluation of Total Antioxidant Capacity in Serum and Follicular Fluid of Women Undergoing ICSI and its Association with Implantation Failure
Purpose: Total antioxidant capacity (TAC) in women serum and follicular fluid (FF) which surrounding
oocytes may be related to the implantation failure .Therefore, we herein examined the relationship between
total antioxidant capacity status in serum and FFand it is association with implantation failure
Method: One hundred and seventeen of non-reproductive women who underwent intra-cytoplasmic sperm
injection (ICSI)included in this study and conducted between March 2018 and April 2019 in Kamal ALSamarai
Hospital, center of fertility and IVF. Serum and follicular fluid were collected from non-reproductive
women aged ranged 20-45 years and BMI (ranged 21.9-36.3kg/m2), TAC were measured using sandwich
ELISA in serum and follicular fluid specimen of 21 women of successful implantation compared to 96
experienced implantation failures.
Results: TAC was increased in serum of implantation failure compared to successful but not significant
differences between two study groups. Whereas TAC levels were highly significant (P=0.002) inFF
of women who had successful implantation (1.08 ± 0.64mmol/L) whereas in failure were lower(0.55 ±
0.42 mmol/L). In addition to that, when evaluating the frequency of TAC category in FF revealed highly
significant differences (P=0.003) between two groups, the majority of failure groups(84.4%) had low TAC
compared with (40%) in successful groups, while a significant increase of sufficient TAC in successful than
failure groups (50% versus 6.1% respectively), whereas the borderline TAC were (9.1% versus 10%) in
failure and successful groups.
Conclusions: TAC in FF may be potential markers for implantation successful in ICSI cycle.