Molecular Detection of Pseudomonas Aeruginosa Isolated from Chicken Cans in the Markets of Al-Muthanna Province
Food safety is very necessary for the general health of the consumer and protection from disease, as well as
the importance of economic sustain ability of the agricultural food sector. The consumer wants to make sure
that the food is safe and that it is not contaminated with bacteria. Therefore, these tests must be performed
to detect bacteria and ensure that food safety.
120 samples was collected from frozen chicken cans from the markets of Al-Muthanna province, using the
sterile plastic bags and after isolation the results showed that (55) sample contain bacteria Pseudomonas
aeruginosa from the total samples above.
The results of bacterial culture were positive in 45 samples whereas the results of direct molecular
identification were positive in 55 samples.
Selective media used for bacteria identification such as MacConkey Agar, Methyle red which has been given
the proportion of isolation (15% and 85% respectively) also some isolates showed their ability to liquefy
gelatin (65%) and fermented sugar lactose (100%) . however biochemical testing also used for all bacterial
isolates for confirm diagnoses.
Polymerase chain reaction technique used for detection bacteria by specific primers sequence the result
appear 45% of total sample contamination by P. aeruginosa.
The genome of P. aeruginosa is relatively large so encodes a large proportion of regulatory enzymes
important for metabolism and efflux of organic compounds. This enhanced coding capability genome allows
for great metabolic versatility and high adaptability to environmental changes,
According our result we recommend food factories to use sterilized materials more accurately and effectively
to store frozen chicken cans.