Molecular Detection of Some Virulence Genes in Stahpylococcusaureus Isolated from Different Human Clinical Specimens

Authors

  • Khaleid Yassen AL-Zamily1, Niran Kadhim F. AL-Rubaey2, Jundi Alak Mahdi Al-Buhilal3

DOI:

https://doi.org/10.37506/mlu.v20i2.1203

Keywords:

Staphylococcus aureus, virulence genes, molecular detection, enterotoxine

Abstract

Staphylococcus aureus is a significantbacteria in numerous simple and more severe diseases of humans
because it possessesmanyvirulence factors which controlled byvarious genes that promoteinvention of the
host cells.
A total of Ninetyisolates of Staphylococcus aureus obtained from various human clinical specimens
distributed asurine samples (30/90) (33.3%), wound swabs (28/90) (31.1%), blood samples (24/90) (26.7%)
and sputum swabs (8/90) (8.9%). The clinical specimens were submitted to diagnostic microbiology
laboratories of Al-Hillah Teaching Hospital,Babylon maternity and children’s Hospital and AL-Mahaweel
General Hospital during the interval from April to September 2019, in Babylon Governorate, Iraq.
The multiplex PCR assay was achieved to identify Fourselected virulence genes such as (sea, seb, eta and
tst) in S. aureus strains by using specific primers and depending from sizes of the products PCR amplicons.
Each90 isolates of S.aureus in human clinicalspecimens were examined.T he results revealed that,the most
frequent gene is sea 74 (82.2%), followed by seb gene 71(78.9%), tstgene 44 (48.9%) and etagene 32
(35.6%) was reported as the least frequent detected gene.
Furthermore, it was found that, the predominancetoxin genes represent the highestrate in S.aureus strains
isolated from wound swabs 85 (38.5%), followed by bloodsamples 69(31.2%), urine samples 54(24.4%) and
finally the sputum swabs 13 (5.9%).

Author Biography

Khaleid Yassen AL-Zamily1, Niran Kadhim F. AL-Rubaey2, Jundi Alak Mahdi Al-Buhilal3

1Assist Prof. Dr. Department of Medical Laboratories Techniques, Kut Technical Institute, Middle Technical
University, Baghdad, Iraq, 2Lect. Dr. Department of Medical Education, Hammurabi College of Medicine,
University of Babylon, Babylon, Iraq, 3Assit prof. Dr. Institute of Medical Technology Al-Mansour,Middle Technical
University, Baghdad, Iraq

Published

2020-05-22

How to Cite

Khaleid Yassen AL-Zamily1, Niran Kadhim F. AL-Rubaey2, Jundi Alak Mahdi Al-Buhilal3. (2020). Molecular Detection of Some Virulence Genes in Stahpylococcusaureus Isolated from Different Human Clinical Specimens. Medico Legal Update, 20(2), 7410-746. https://doi.org/10.37506/mlu.v20i2.1203

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