Viability Testof Lemongrass Extract (Cymbopogon Citratus) on BHK-21 Fibroblast Cell Culture

Authors

  • Shafira Kurnia Supandi1, Yoshua Christian Michael1, Lambang Bargowo1

DOI:

https://doi.org/10.37506/mlu.v20i2.1217

Keywords:

Extract lemongrass, BHK-21 fibroblast cells, viability.

Abstract

Background: Lemongrass are found in Indonesia thus it lemongrass can be used as an alternative in healing
inflammation by conducting a preliminary study in the form of BHK-21 fibroblast cell viability test after 24
hours of extract lemongrass.
Objective: To determine the viability of extract lemongrass against BHK-21 fibroblast cell cultures. Method:
BHK-21 fibroblast cells were taken from neonatal hamsters’ kidneys and planted in 96 well plates. Extracts
lemongrass with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.13% and 1.56% were included in
each well prepared and incubated for 24 hours. MTT Assay was performed to see the viability of extract
lemongrass on fibroblast cells.
Results: Extract lemongrass viable against BHK-21 fibroblast cells at a concentration of 100%. There is a
decrease in viability at concentrations of 50% to 1.56%.
Conclusion: The 100% concentration showed the highest viability of BHK-21 fibroblast cells and extract
lemongrass had potential in wound healing.

Author Biography

Shafira Kurnia Supandi1, Yoshua Christian Michael1, Lambang Bargowo1

1Department of Periodonsia Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Mayjen,
Prof. Dr. Moestopo Street, Surabaya, Indonesia, 60132

Published

2020-05-22

How to Cite

Shafira Kurnia Supandi1, Yoshua Christian Michael1, Lambang Bargowo1. (2020). Viability Testof Lemongrass Extract (Cymbopogon Citratus) on BHK-21 Fibroblast Cell Culture. Medico Legal Update, 20(2), 820-824. https://doi.org/10.37506/mlu.v20i2.1217

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