Viability Testof Lemongrass Extract (Cymbopogon Citratus) on BHK-21 Fibroblast Cell Culture
Keywords:Extract lemongrass, BHK-21 fibroblast cells, viability.
Background: Lemongrass are found in Indonesia thus it lemongrass can be used as an alternative in healing
inflammation by conducting a preliminary study in the form of BHK-21 fibroblast cell viability test after 24
hours of extract lemongrass.
Objective: To determine the viability of extract lemongrass against BHK-21 fibroblast cell cultures. Method:
BHK-21 fibroblast cells were taken from neonatal hamsters’ kidneys and planted in 96 well plates. Extracts
lemongrass with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.13% and 1.56% were included in
each well prepared and incubated for 24 hours. MTT Assay was performed to see the viability of extract
lemongrass on fibroblast cells.
Results: Extract lemongrass viable against BHK-21 fibroblast cells at a concentration of 100%. There is a
decrease in viability at concentrations of 50% to 1.56%.
Conclusion: The 100% concentration showed the highest viability of BHK-21 fibroblast cells and extract
lemongrass had potential in wound healing.