The Effect of Soot Particulate towards Vascular Cell Adhesion Molecule-1 (VCAM-1) Expression in the Mechanism of Cardiovascular System Disruption

  • Edmond Leonard1, Muhammad Aminuddin1
Keywords: Soot particulate, vascular cell adhesion molecule-1 (VCAM-1)

Abstract

Background: Air pollution is associated with cardiovascular morbidity and mortality; however, the
underlying mechanisms are not yet clearly understood. Several previous studies have implicated potential
mechanism action including oxidative stress, systemic inflammation, autonomic dysfunction, and endothelial
dysfunction. Several epidemiological studies have examined the association between ICAM-1, VCAM-1
and particulate matter.
Objective: To describe the effect of soot particulate exposure in VCAM-1 expression in the mechanism of
cardiovascular dysfunctions.
Method: The experiment was conducted in laboratory female rats (Rattus novergicus) and consisted of 3
groups: Control group (n=10), without soot particulate exposure; Treatment 1 group (n=12), exposed by
soot particulate with the concentration of 532 mg/m3 an hour each day for 30 days; Treatment 2 group
(n=12), exposed by soot particulate with the concentration of 1064 mg/m3 an hour each day for 30 days.The
expression of VCAM-1 on cardiac tissue was measured after the end of treatment by immunohistochemical
examination. The differentiation of VCAM-1 expression among the groups was tested using the Kruskal-
Wallis test and the Mann-Whitney test.
Results: The mean rank of VCAM-1 expression in the control group, treatment group 1 and treatment group
2 was significantly different (8.85, 17.63, 24.58, p=0.001). There was a significant difference in VCAM-1
expression by using the Mann-Whitney test among groups (p <0.05).
Conclusion: The exposure to soot particles increased VCAM-1 expression significantly in laboratory
animals. Our findings indicated the important role of the inflammatory activation pathway as a response to
soot particulate exposure in the mechanism of cardiovascular disease.

Author Biography

Edmond Leonard1, Muhammad Aminuddin1

1Department of Cardiology and Vascular Medicine, Faculty of Medicine Universitas Airlangga,
Dr. Soetomo Teaching Hospital, Surabaya 60285, Indonesia

Published
2020-05-22
How to Cite
Edmond Leonard1, Muhammad Aminuddin1. (2020). The Effect of Soot Particulate towards Vascular Cell Adhesion Molecule-1 (VCAM-1) Expression in the Mechanism of Cardiovascular System Disruption. Medico Legal Update, 20(2), 830-835. Retrieved from http://ijop.net/index.php/mlu/article/view/1219