Antibacterial Activity of Selected Fractions Extract of Coptis chinensis Franch Rhizomes

Authors

  • Reyadh R Al-Rashidi1 , Hisham Al-Shaikhli2 , Rusul A Alsaray1 , Tahrani Munusamy3 , Radhi K. Obaid4

DOI:

https://doi.org/10.37506/mlu.v20i3.1531

Keywords:

Coptis chinensis, Fractions, Antibacterial activity, Zone of inhibition

Abstract

The antibacterial activity of different fractions of selected extract of Coptis chinensis Franch rhizome

was evaluated on gram positive bacteria. Three extracts were tested at concentrations ranging from 1 mg/

ml, 10 mg/ml, and 100 mg/ml in vitro on three bacterial strains, Staphylococcus aureus, Streptococcus

pneumoniae, and Bacillus anthracis, which was done by performing disc diffusion for growth inhibition. The

methanol extract at concentrations of 100 mg/ml displayed the most significant zone of inhibition of (22.67

± 3.055mm), (21.67 ± 3.512 mm), (16.67 ± 1.528 mm) against Streptococcus pneumoniae, Staphylococcus

aureus, and Bacillus anthracis, respectively. The TLC separation of the extracts showed that each of them may

contains constituents that are phenolic in nature. From the Rf values of the spots found in the various extracts

is that the Coptis chinensis Franch rhizome contains some other non-phenolic compounds responsible for its

effect. The selected methanol extract is then subjected to column chromatography and collected 4 fractions.

Out of the four fractions, F2 fraction demonstrates the most significant zone of inhibition of 15.00 ± 1.000

mm, 12.33 ± 1.528 mm and 10.00 ± 2.000 mm against Streptococcus pneumoniae, Staphylococcus aureus,

and Bacillus anthracis, respectively.

Author Biography

Reyadh R Al-Rashidi1 , Hisham Al-Shaikhli2 , Rusul A Alsaray1 , Tahrani Munusamy3 , Radhi K. Obaid4

1 Assist. Lecturer/ Department of Dentistry, Alkut University College, 52001, Wasit, Iraq, 2 Lecturer/ School of

Public health and Interdisciplinary Studies, Faculty of health and environmental sciences, Auckland University

of Technology, 1142, Auckland, New Zealand, 3 Researcher/ School of Pharmacy, Management and Science

University, 40100, Shah Alam, Selangor Darul Ehsan, Malaysia, 4 Prof/ Department of Pharmacy, Alkut University

College, 52001, Wasit, Iraq

Published

2020-07-24

How to Cite

Reyadh R Al-Rashidi1 , Hisham Al-Shaikhli2 , Rusul A Alsaray1 , Tahrani Munusamy3 , Radhi K. Obaid4. (2020). Antibacterial Activity of Selected Fractions Extract of Coptis chinensis Franch Rhizomes. Medico Legal Update, 20(3), 985-990. https://doi.org/10.37506/mlu.v20i3.1531

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