Molecular detection of Virulence Factors genes Associated with  Immune Resistance in Klebsiella pneumonia

Authors

Klebsiella pneumonia, molecular detection, virulence gene.

The current study was aimed to genotypically detection of genes encode outer membrane proteins related

with human serum resistance. A total of 33 isolates were identified as Klebsiella pneumonia according to the

manual cultural characteristics and biochemical tests and then confirmed by Vitek -2 system. The number

of isolates according to site of infection were 14 (42.4%) isolates from wound infection, 15(45.4%) isolates

from urine, 1(3.03%) isolates from blood, 1(3.03%) isolates from vagina,1(3.03%) from ear swab and finally

1(3.03%) isolates from stool. The present study demonstrated a positive relationship between the ability

of bacterial isolates to antibiotics resistance and the occurrence of some virulence genes. The molecular

detection of some virulence genes (htrA, rmpA, mrkA and iss) was done by conventional PCR technique.

It was found that ,high occurrence of htrA gene was observed among Klebsiella pneumonia isolates which

was found in 26(78% ) of isolates at 1071bp , then 17(51%) isolate were positive for rmpA gene at 967

bp,whereas mrkA gene was recorded in 5(15.15%) isolates at 862 bp , and only 4(12.12%) isolates gave

positive result for iss gene at 450bp.

1 M.Sc.,2 Prof. Dr., 3 Assist.Prof. Dr., Department of Biotechnology, College of Biotechnology, Al-Qasim Green

University, Babylon, Iraq