The Effect of Competitive Antigen on Some Cytokine in  Rabbits

Authors

Competitive antigen, Cytokine, Rabbits, Effect

Bacterin can be divided into dead bacterin and live bacterin. Dead bacterin is a virulent strains which

have a good immunogenicity .the aim of this study to know competitive between two bacterin E.coli and

P.aeruginosa in different concentrations .The rabbits were used as model for this study and the rabbits were

divided into six groups each groups composed 5 rabbits , After immunized programs .the blood were collected

. The sera were separated and used to determine TNF-? ,IL-2 and IL-10 by enzyme linked immunosorbent

assay .the results appeared .The results appeared different count of bacteria increased (54.09, 68.33)TNF-?

compared with control(42.55) . the concentrations of Il-2 were increased with increased dose of E .coli

(16.90, 20.29)also the same occurred with P .aeruginosa (in P aeruginosa alone was15.14 while combined

with E.coli 15.56 and15.38 ,the results appeared the means of IL-2 concentrations were higher, Interleukin

10 were showed its mean was increased in same concentrations of bacterin but decreased in different count .

The present findings seems to be novel since combined bacterins have the potential for use as an autogenous

bacterins in therapy for cases of complicated multi-drug resistant infections like that of urinary tract.

1 M.Sc., Student, 2 Prof. Dr., Department of Biotechnology, College of Biotechnology, University of Qasim, Qasim

Babylon, Iraq, 3 Prof. Dr., Department of Biology, College of Science, University of Babylon