Production of Bioemulsifiers from Bacteria Grown on Hydrocarbons


  • Ghassan M. Ahmed



Biosurfactants, emulsifiers, soil flora, hydrocarbon contamination


This work aimed to identify biosurfactant-producing bacterial strains isolated from hydrocarbon-contaminated
sites and to evaluate their biosurfactant properties. The drop-collapse method and minimal agar added with
a layer of diesel as carbon source were used for screening, positive strains were able to grow in a liquid
medium, and surface tension and emulsification index were determined in cell-free supernatant and cell
suspension. 250 bacterial isolate were tested, and 16 were positive for the drop-collapse and hydrocarbonlayer agar method. Most of the strains were Pseudomonas, except for three strains (Acinetobacter, Bacillus,
and Rhodococcus). Surface tension was similar in cell-free and cell suspension measurements, with values
in the range of 58 to 26 (mN/m), and all formed stable emulsions with engine oil (77-92% E24). Considering
the variety of molecular structures among microbial biosurfactants, they have different chemical properties
that can be exploited commercially, for applications as diverse as bioremediation or degradable detergents.

Author Biography

Ghassan M. Ahmed

Assistant Lecture, Biotechnology Department/Institute of Genetic Engineering and Biotechnology for
Graduate Studies/University of Baghdad



How to Cite

Ghassan M. Ahmed. (2021). Production of Bioemulsifiers from Bacteria Grown on Hydrocarbons. Medico Legal Update, 21(1), 275-279.