The Effect of Qourum Sensing genes (lasI, rhlI) in Some Virulence Factors of Pseudomonas aeruginosa Isolated From Different Clinical Sources

Authors

  • Hasan Falah Lahij1, Abdulla H. Alkhater2 , Muthanna Hamid Hassan1, Luma Amer Yassir3

DOI:

https://doi.org/10.37506/mlu.v21i1.2325

Keywords:

Pseudomonas aeruginosa, Quorum sensing, virulence factors, Biofilm, Pyocyanin.

Abstract

Background and Objective: Quorum sensing (QS) is a chemical language of bacteria, and use of N-acylhomoserine lactone (AHL) signals is one of the most known mechanisms by which bacteria can communicate
with each other to regulate the gene expression based on bacterial density. Previous studies suggested that QS
has an important role in pathogenicity of P.aeruginosa, so that the present study investigation the correlation
of QS genes in local isolate of P.aeruginosa with production of virulence factors.
Materials and Method: The study included collection of 126 samples from various clinical sources and,
these samples included wounds, burns, ear, cystic fibrosis and urinary tract infections (UTI). Pyocyanin
and biofilm production were determined spectrophotometrically, QS genes were detected by PCR based on
specific sequences for lasI and rhlI genes.
Results: Final diagnosis showed that 51 isolates belong to target bacteria and, the phenotypic detection
of some virulence factors showed that 46 isolates (90%) showed variance in the strength of Pyocyanin
production, and 44 (86%) of the isolates showed a differences in their ability to biofilm formation and,
screed QS genes showed that 48 (94%) of isolates were positive for lasI gene, while 44 (86%) of isolates
have rhlI gene, and this study pointed that 9 isolates have one of QS genes lasI or rhlI, and also have one or
more of virulence factors, as well as the results showed that 1 isolate out of 51 negative for both QS genes
and weakly in production of virulence factors.
Conclusion: Most of local isolates were positive for QS genes and, QS plays an important role in the
pathogenesis, not all of the virulence factors controlled by QS. The study indicated that the isolates which
have both QS genes were more virulence than isolates that have single gene.

Author Biography

Hasan Falah Lahij1, Abdulla H. Alkhater2 , Muthanna Hamid Hassan1, Luma Amer Yassir3

1Lecturer, Biology Department-Collage of Sciences, University of Anbar, Ramadi, Iraq, 2Scholar researcher,
Al-Maarif University Collage, Department of Medical Laboratory Techniques, Iraq, 3
Prof, National Center of
Hematology, Al -Mustansiryah University, Baghdad, Iraq

Published

2021-01-09

How to Cite

Hasan Falah Lahij1, Abdulla H. Alkhater2 , Muthanna Hamid Hassan1, Luma Amer Yassir3. (2021). The Effect of Qourum Sensing genes (lasI, rhlI) in Some Virulence Factors of Pseudomonas aeruginosa Isolated From Different Clinical Sources. Medico Legal Update, 21(1), 303-308. https://doi.org/10.37506/mlu.v21i1.2325