Molecular Profile of Integrase gene intI and Carbapenem gene in Aeromonas sobria Isolates

Authors

  • Hawraa Natiq Kabroot AL-Fatlawy1 , Taghreed Abdul Al-Kareem Al-Makhzoomy2 , Mohammed Abdulaali Al-Hakeem3

DOI:

https://doi.org/10.37506/mlu.v21i1.2327

Keywords:

Aeromonas soria, IMP gene, integrase intI1, Antibiotics and imipenem.

Abstract

The current study includes the detection of Aeromonas sobria isolates were obtained from total 157 samples
involved the clinical samples (diarrhea samples) from patients suffering from diarrheal infections during the
period from April 2017 to November 2017. at AL-Central Health Laboratory in Najaf governorate. These
isolates were diagnosed by four method as (Culture method, biochemical tests, Vitek@2GN cards system
and Polymerase chain reaction (PCR) technique). TheVitek@2GN cards system was the best method for
diagnosis, which have led to isolate and diagnosis of 33 isolate. Multidrug resistance (MDR) of bacteria
were detected by MIC testing and performed with the automated VITEK@2 GN/AST compact system.
A clear variation was observed in their susceptibility to 13 antibiotics disks, these isolates of A.sobria
were revealed resistance for some antibiotics such as Penicillin class Methicillin, Amoxicillin, Ampicillin,
Penicillin (100%), and (91.6%) for Carbenicillin, and high rates of resistance (100%) to Cephalosporins
that represented by Ceftazidime, Cefotaxime, Cefoxitin and Cefepime. The investigation of mobile genetic
elements among isolates demonstrated thatA.sobria have been Class I integron genes (class 1 integron
represented by integrase intI1, and IMP genes).

Author Biography

Hawraa Natiq Kabroot AL-Fatlawy1 , Taghreed Abdul Al-Kareem Al-Makhzoomy2 , Mohammed Abdulaali Al-Hakeem3

1Ph.D. Dr. Academic(Molecular Genetics) in Dep. of Medical Laboratory Techniques, Al-Toosi University College,
Najaf, IRAQ, 2
M.Sc.(Microbiology) in Laboratory Investigation department, Faculty of Science, University of
Kufa, Najaf, IRAQ. 3B.Sc. in Biology department, Faculty of Science, University of Kufa, Najaf, IRAQ

Published

2021-01-09

How to Cite

Hawraa Natiq Kabroot AL-Fatlawy1 , Taghreed Abdul Al-Kareem Al-Makhzoomy2 , Mohammed Abdulaali Al-Hakeem3. (2021). Molecular Profile of Integrase gene intI and Carbapenem gene in Aeromonas sobria Isolates. Medico Legal Update, 21(1), 316-320. https://doi.org/10.37506/mlu.v21i1.2327

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