The ITGB3 Genevariant among Sample of Glanzmannthrombasthenia Iraqi Patients

Authors

  • Hayderkareemkataa AL-Aidy1 , Ehab Dawood Salman2 , Safa A Faraj3 , Zaid Jamal Mahmood4

DOI:

https://doi.org/10.37506/mlu.v21i1.2330

Keywords:

Glanzmann’sthrombasthenia, ITGB3 Gene Polymorphisms, rs115310198,rs2317676,rs3809865.

Abstract

Case control study was used,with Glanzmann’s thrombasthenia (GT) patients (n=15) and healthy individual
control (n=20).
It was successfully identified three SNP in ITGB3 gene by using PCR technique and direct sequencing . The
first SNP c. * 1479T> C (rs115310198) was presented with three genotypes (TT, TC and CC). The genotype
frequencies of TT in control group (87.6 vs. 95 %) show non-significant difference (p>0.05) compared with
GT patients . It was also noticed the frequency of mutant allele (C) revealed non-significant difference (p >
0.05) in GT patients compared with controls group (13.3 vs. 2.5%; OR = 6; EF = 0.03; 95% C.I. = 0.65 to
55.05) that mean (C) allele is risk factor associated with GT patients .
The c.*713A>G (rs2317676) was given with three genotypes (AA, AG and GG) . The frequencies of these
genotypes show non-significant difference (p>0.05) between control and GT patient,while the mutant allele
(G) show non-significant difference (p > 0.05) in GT patients compared with controls group (16.6 vs. 5%;
OR = 3.8; EF = 0.4; 95% C.I. = 0.70 to 20.63) and it was risk factors of patient GT. The c.*1016T>A
(rs3809865) showed three genotypes (TT, TA and AA) with two alleles (T and A). The frequencies of these
genotypes TT (87.6 vs. 85 %), TA (6.6vs. 15 %) and the third genotype AA (6.6 vs. 0%) show non-significant
difference (p>0.05) between control and GT patient but the genotype (AA) (OR= 4.24) was considered as
risk factors with GT patients . It was also found the mutant allele (A) revealed non-significant difference (p
> 0.05) in GT patients compared with controls group (10 vs. 7.5%;OR = 1.37; EF = 0.11; 95% C.I. =0.26 to
7.14). (A) allele(OR = 1.37) is positively associated with GT patients and negatively associated with healthy
subject .

Author Biography

Hayderkareemkataa AL-Aidy1 , Ehab Dawood Salman2 , Safa A Faraj3 , Zaid Jamal Mahmood4

1
MSC Molecular Biology, Wassit Health Department, Ministry of Health, Iraq, 2Ph.D Molecular Biology
Department of Biotechnology, College of Science, University of Baghdad, Iraq, 3
MD Pediatrics Hema-oncologist,
Wasit University, College of Medicine, Iraq, 4M.B.Ch.B . FIBMS, Congenital Bleeding, Children Welfare Hospital,
Medical City

Published

2021-01-09

How to Cite

Hayderkareemkataa AL-Aidy1 , Ehab Dawood Salman2 , Safa A Faraj3 , Zaid Jamal Mahmood4. (2021). The ITGB3 Genevariant among Sample of Glanzmannthrombasthenia Iraqi Patients. Medico Legal Update, 21(1), 333-339. https://doi.org/10.37506/mlu.v21i1.2330

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