Appraisal of Genotoxicity and Cytotoxicity of Depakine® and/ or Epanutin® in Bone Marrow Erythrocytes and Hepatocytes of Male Albino Mice by Comet and Micronucleus Assays

Authors

  • Nagla Zaky Ibrahim El -Alfy1 , Amany Ibrahim Alqosaibi2 , Mahmoud Fathy Mahmoud3, Ayman Mohammad Abdullah4

DOI:

https://doi.org/10.37506/mlu.v21i1.2384

Keywords:

Epilepsy, liver, mutagenicity, phenytoin, sodium valproate.

Abstract

Background: Depakine and epanutin are widely used in the treatment of a variety of diseases. A special
attention should be given for their deleterious impacts on genetic material of patients.
Method: Sixty-five male albino mice were separated into four main sections. First is control injected with
normal saline 1ml/kg, second section injected with depakine 25 & 50 mg/kg/day, third section injected with
epanutin 3 & 6 mg/kg/day and fourth section injected with (depakine 25 + epanutin 3) & (depakine 50 +
epanutin 6) mg/kg/day. Injections were via intraperitoneal, samples of both bone marrow erythrocytes for
micronucleus test and hepatocytes for comet assay were collected after one and two weeks for each dose.
Conclusions: Human therapeutic doses range of depakine and/or epanutin generate (s) variable degrees
of damages in genetic material in both bone marrow erythrocytes and hepatocytes of male albino mice
depended on dose and time

Author Biography

Nagla Zaky Ibrahim El -Alfy1 , Amany Ibrahim Alqosaibi2 , Mahmoud Fathy Mahmoud3, Ayman Mohammad Abdullah4

1 Professor of Cytogenetics, 2Assistant Professor of Molecular genetics, Department of Biology, College of
Science, University of Imam Abdulrahman bin Faisal, P. O. Box 1982, Dammam, 31441, Saudi Arabia, 3
Professor
of Cytology and Histology, 4PhD Researcher, Department of Biological and Geological Sciences, Faculty of
Education, Ain Shams University, Cairo, Egypt

Published

2021-01-09

How to Cite

Nagla Zaky Ibrahim El -Alfy1 , Amany Ibrahim Alqosaibi2 , Mahmoud Fathy Mahmoud3, Ayman Mohammad Abdullah4. (2021). Appraisal of Genotoxicity and Cytotoxicity of Depakine® and/ or Epanutin® in Bone Marrow Erythrocytes and Hepatocytes of Male Albino Mice by Comet and Micronucleus Assays. Medico Legal Update, 21(1), 638-643. https://doi.org/10.37506/mlu.v21i1.2384