Antibiofilm Activity of Nystatin, Aspirin and EDTA Against Candida albicans Isolated from Iraqi Women with Vulvovaginitis
Keywords:Nystatin, Aspirin, EDTA, C. albicans, Biofilm, Vulvovaginitis.
The vulvovaginitis candidiasis is often associated with biofilm formation by Candida albicans and using of
antifungal agents against C. albicans biofilms is urgently needed. Microtiter plate assay using crystal violet
was used for detection the ability of Candida albicans to form biofilm and the microtiter broth dilution
method was used for determination the minimum inhibitory concentrations (MICs). Out of 42 Candida
albicans isolated from vulvovaginitis, 37 (88%) can produce biofilm at the varying degrees. Twenty-eight
(75.7%) isolates could form a strong biofilm. The results of minimum inhibitory concentrations (MICs) of
Nystatin, Aspirin and EDTA (Ethylenediaminetetraacetic acid) against 28 C. albicans isolates which formed
the strong biofilm, revealed that range of concentrations of Nystatin were (6.25-100 µg/ml), while the MICs
of aspirin and EDTA were more than 1000 µg/ml. It was obvious that the Nystatin had the inhibitory activity
at the concentrations 6.25 and 12.5 µg/ml. The highest antibiofilm activity by Nystatin were demonstrated
at the subinhibitory concentration 50 ?g/ml with biofilm eradication percent (75.80%), while the lowest
antifungal effect (2.86-10.70%) was at very low concentrations (3.125-6.25 ?g/ml). Also, there was an
obvious biofilm eradication of Aspirin and EDTA at the concentrations 500 and 1000 ?g/ml but the effect
of aspirin at the concentration 1000 ?g/ml (70.51%) is more than EDTA (60.12%) in contrast with the
concentration 500 ?g/ml, it was found that the effect of EDTA (51.29%) is more than aspirin (34.25%). In
conclusion, the present study highlights the role of Aspirin and EDTA as antibiofilm agents when used with
Nystatin which have the ability to inhibit the growth of C. albicans in patients with vulvovaginitis.
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