Study ndvB gene expression in Pseudomonas aeruginosa Producing Biofilm
Keywords:Pseudomonas aeruginosa, ndvB, biofilm
Pseudomonas aeruginosa have ability to form biofilm, this biofilm responsible for wide infections and
antibiotic resistance development. A novel mechanism to antibiotic resistance mechanisms utilized by
biofilm represented by the presence of ndvB geneencodes a glucosyltransferase enzyme involved in the
formation of this glucans, thisglucan mediated sequestration antibiotic away from their cellular targets.
In this study was collected 145 samples from burns patients and identification by biochwmicaltsets and
Vitek-2 compact. The studied the biofilm formation was measured using micro titer plate method we found
that 49 isolates (100%) of P.aeruginosa were biofilm positive and measured minimal biofilm inhibitory
concentration(MBIC) of biofilm P.aeruginosa positive biofilmused cefepime as antibiotic was tested using
microtiter plate microdilution method (MBIC) values (1gm/ml).Molecular detection of ndvB gene was
measured using PCR 100% of isolates were found to contain ndv B gene. Expression of ndvB gene was
significantly high in biofilm isolates using sub- MBIC values of cefepime. Expression of ndvB gene was
significantly high in biofilm isolates using glucose with concentration 1% and 2%. This study we concluded
biofilm formation is an important trait of P.aeruginosa that isa cause of antibiotic resistance. ndvB gene
expression responsible for the biofilm resistance mechanism in P. aeruginosa biofilms.
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