Synthesized Gold Nanoparticles Using Pseudomonas Supernatant and Study the Physical Characterization– Antproliferative Activity of Breast Cancer Cells (MCF-7)
Keywords:Gold Nanoparticles, MCF-7, Anticancer
In this paper we used a biological method to prepare gold nano particles as a simple technique, environmentally
friendly and limited toxics compared with chemical and physical method. Where used Pseudomonas bacteria
supernatant to prepare gold nano particles and use this supernatant to reduction gold salt (HAuCl4.4H2O)
and converted to AuNPs. XRD measurement showed the average the crystallite size ranging from (9.2-11.4)
nm, UV-VS spectrum exhibited the maximum absorbance peak at (530)nm and optical energy gap (Eg =
1.88)e.v, FESEM image showed the shapes of AuNPs as (spherical and semispherical), TEM image showed
the formation of particles as clusters, spherical and hexagonal shapes. AuNPs was used as anticancer cell
line where testing against breast cancer line (MCF-7) and interested result indicated that can be used AuNPs
in reliably as an active treatment to those have breast cancer (MCF-7).
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