Detection of Legionella pneumophila and Legionella dumoffii Biochemically in Water Samples in Baghdad City, Iraq
Keywords:Legionella pneumophila, Legionelladumoffii, Water sources, Antibiotic resistance
Background: Legionellae can be isolated from various sources of water. Legionella pneumophila is
transmitted via contaminated water and caused many diseases like pneumonia; lungs abscesses and fever.
Objectives: Isolation and identification of L. pneumophila from environmental samples; study the factors
that affect the frequency of L. pneumophila; and some oxidizing substances on their growth.
Materials and Method: A total of one hundred water samples were collected from Cooling tower; Water
tanks; Tap water and Swab from inner tap water. All samples were cultured on Buffered charcoal yeast extract
(BCYE) agar and Buffered charcoal yeast extract (BCYE) broth to isolate Legionella pneumophila and
Legionella dumoffii. Effect of pH; temperature; chlorine; Iron and effect of soluble zinc on L. pneumophila
growth were studied.
Results: The isolates gave positive results for the tests of catalase, oxidase, gelatin degradation, nitrate
reduction and beta-lactamase. Iron; Soluble Zinc; Chloride; Calcium hardness (CaCO3); Turbidity and silver
were ? 3ppmas; 1-3 ppmas; 0.2-0.5 ppm; 600 ppm; ? 0.3 and 3 ppmas respectively. The distribution of L.
pneumophila in the water samples were studied.
Conclusion: FatalL. pneumophila may be transmitted by drinking contaminated water and led to death
because they caused pneumonia.
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