Molecular Study of Genetic Diversity in Escherichia coli Isolated from Tap Water in Baquba

Authors

  • Noor Jameel Allhaby1 , Hadi Rahman Rasheed Al-Taai2

DOI:

https://doi.org/10.37506/mlu.v21i1.2506

Keywords:

E. coli, ERIC-PCR, BOX-PCR, RAPD–PCR.

Abstract

One hundred and ten samples of the tap water in Baquba city were collection .Ten isolates were obtained.
The results of the sensitivity test showed that 10 isolates were multidrug, 10 isolates exhibited moderate
resistance ranging from 5-8 antagonists. Through the use of the RAPD-PCR profiling system, the vast
majority of Escherichia coli strains under study have large genomes and high virulence factors by belonging
to group A2. Results of genetic profiling using the ERIC- PCR and BOX-PCR system showed several groups
of isolates were identified as having a genetic variation and B2 group was given the highest proportion
of ERIC- PCR system,70% in ERIC While A2 group was given the highest proportion of BOX- PCR
system,40% in the BOX indicating that there is a negative spread The present study indicates that there is a
correlation between taxonomic systems was evident by the distribution of isolates in each pattern and group.

Author Biography

Noor Jameel Allhaby1 , Hadi Rahman Rasheed Al-Taai2

1Postgraduate, 2Prof. Dr., College of Science, Diyala University, Iraq

Published

2021-01-09

How to Cite

Noor Jameel Allhaby1 , Hadi Rahman Rasheed Al-Taai2. (2021). Molecular Study of Genetic Diversity in Escherichia coli Isolated from Tap Water in Baquba. Medico Legal Update, 21(1), 1338-1344. https://doi.org/10.37506/mlu.v21i1.2506