Detection of Anti-GAD65 Antibodies in Sera of Diabetic Patients Using a Home-Made Latex Agglutination Kit

Authors

  • Alaa S. Alattabi1 , Dhamiaa M. Hamza1 , Khalid Khalil Alaaraji1 , Sawsan M. Jabbar1 , Abeer Thaher Naji AL-Hasnawi1 , Ali Mansoor Jasim1

DOI:

https://doi.org/10.37506/mlu.v21i1.2555

Keywords:

Homemade latex kit, Anti-GAD56 antibody, LADA

Abstract

Objectives: Compare sensitivity of anti-GAD65 result detected by ELISA with that one detected by homemade latex agglutination kit.
Patients and Method: Thirty seven patients were enrolled in this study from January, 2019 to March,
2019. The sample had been taken from patients attended diabetic unit in Imam Hussain medical city in
kerbala. Informed consent was taken from each participant in the study. Serum samples were tested by
ELISA technique (GAD65 ELISA Kit, CUSABIO BIOTECH CO., LTD. USA) and by home-made latex
agglutination for anti-GAD 65.
Results: Data from the current study showed that readings of the home-made latex test are consistent with
those detected by ELISA as a confirmatory test. When analyzed, results showed sensitivity 94.44%specificity
78.95%, positive predictive value 80.95,negative predictive value 3.75%, positive likelihood 4.49, negative
likelihood 0.07.
Conclusion: This home-made latex agglutination kit sensitive as ELISA could be used as a reliable test to
detect anti GAD65 in diabetic patients.

Author Biography

Alaa S. Alattabi1 , Dhamiaa M. Hamza1 , Khalid Khalil Alaaraji1 , Sawsan M. Jabbar1 , Abeer Thaher Naji AL-Hasnawi1 , Ali Mansoor Jasim1

1
Assist. Prof., Kerbala University, College of Medicine

Published

2021-01-09

How to Cite

Alaa S. Alattabi1 , Dhamiaa M. Hamza1 , Khalid Khalil Alaaraji1 , Sawsan M. Jabbar1 , Abeer Thaher Naji AL-Hasnawi1 , Ali Mansoor Jasim1. (2021). Detection of Anti-GAD65 Antibodies in Sera of Diabetic Patients Using a Home-Made Latex Agglutination Kit. Medico Legal Update, 21(1), 1636-1639. https://doi.org/10.37506/mlu.v21i1.2555

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