Effect of Moringa Oleifera Leaf Extract on Oxidative Stress and Theca Cell in Polycystic Ovary Syndrome Model with Insulin Resistance

Authors

  • Lisa Purbawaning Wulandari
  • Budi Santoso
  • Bambang Purwanto
  • Muhammad Miftahussurur
  • Jimmy Yanuar Annas
  • Budiono

DOI:

https://doi.org/10.37506/mlu.v21i2.2771

Keywords:

Moringa oleifera, Polycystic Ovary Syndrome, malondialdehyde, Theca Cell Thickness.

Abstract

Background: The use of Moringa oleifera as an antioxidant should be investigated as an alternative
treatment of oxidative stress and follicular refinement in PCOS with insulin resistance.
Purpose: We aimed to prove the effect of Moringa oleifera leaf extract in various dosages to decrease the
malondialdehyde levels and theca cell thickness of PCOS female rat with insulin resistance.
Method: Three month old Rattus norvegicus stran wistar rat weighing 100-130 grams were divided into 5
groups (n = 8). PCOS model obtained by giving injection of testosterone propionate for 28 days, followed
by metformin therapy and Moringa oleifera leaf extract at 250 and 500 mg/KgBW for 14 days. Then, we
analyzed levels of malondialdehyde in the blood and the thickness of theca cell.
Results: Malondialdehyde levels in the PCOS control group (5.694±1.464) increased significantly (p <0.05)
compared to the normal controls (1.939 ±0.341). Leaf extract Moringa oleifera 500 mg/KgBW (1.982±0.383)
showed a significant decrease (p <0.05) to malondialdehyde levels compared to the PCOS control group.
Examination of ovarium histology showed that leaf extract Moringa oleifera 500 mg/KgBW (0.931±0.457)
significantly decreased the thickness of theca cells (p <0.05) compared to the PCOS control group.
Conclusion: Moringa oleifera leaf extract as an antioxidant proven to decrease the malondialdehyde levels
and the thickness of theca cell of the female rat model of PCOS.

Author Biographies

Lisa Purbawaning Wulandari

Researcher of School of Reproduction Health, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Budi Santoso

Lecturer of Fertility and Reproductive Endocrinology Division, Department of Obstetrics and Gynecology,
Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya, Indonesia

Bambang Purwanto

Lecturer
of Department of Physiology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Muhammad Miftahussurur

Lecturer of
Gastroentero-Hepatology Division, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching
Hospital-Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia

Jimmy Yanuar Annas

Lecturer of Department of
Obstetrics and Gynecology, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas Airlangga, Surabaya,
Indonesia

Budiono

Lecturer of Department of Epidemiology, Faculty of Medicine, Universitas Airlangga, Surabaya,
Indonesia

Published

2021-03-12

How to Cite

Lisa Purbawaning Wulandari, Budi Santoso, Bambang Purwanto, Muhammad Miftahussurur, Jimmy Yanuar Annas, & Budiono. (2021). Effect of Moringa Oleifera Leaf Extract on Oxidative Stress and Theca Cell in Polycystic Ovary Syndrome Model with Insulin Resistance. Medico Legal Update, 21(2), 744-749. https://doi.org/10.37506/mlu.v21i2.2771