Effect of Moringa Oleifera Leaf Extract on Oxidative Stress and Theca Cell in Polycystic Ovary Syndrome Model with Insulin Resistance
Keywords:Moringa oleifera, Polycystic Ovary Syndrome, malondialdehyde, Theca Cell Thickness.
Background: The use of Moringa oleifera as an antioxidant should be investigated as an alternative
treatment of oxidative stress and follicular refinement in PCOS with insulin resistance.
Purpose: We aimed to prove the effect of Moringa oleifera leaf extract in various dosages to decrease the
malondialdehyde levels and theca cell thickness of PCOS female rat with insulin resistance.
Method: Three month old Rattus norvegicus stran wistar rat weighing 100-130 grams were divided into 5
groups (n = 8). PCOS model obtained by giving injection of testosterone propionate for 28 days, followed
by metformin therapy and Moringa oleifera leaf extract at 250 and 500 mg/KgBW for 14 days. Then, we
analyzed levels of malondialdehyde in the blood and the thickness of theca cell.
Results: Malondialdehyde levels in the PCOS control group (5.694±1.464) increased significantly (p <0.05)
compared to the normal controls (1.939 ±0.341). Leaf extract Moringa oleifera 500 mg/KgBW (1.982±0.383)
showed a significant decrease (p <0.05) to malondialdehyde levels compared to the PCOS control group.
Examination of ovarium histology showed that leaf extract Moringa oleifera 500 mg/KgBW (0.931±0.457)
significantly decreased the thickness of theca cells (p <0.05) compared to the PCOS control group.
Conclusion: Moringa oleifera leaf extract as an antioxidant proven to decrease the malondialdehyde levels
and the thickness of theca cell of the female rat model of PCOS.
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