The Association of Gene Expression and Single Nucleotide Polymorphism (rs 6152 SNP) in Androgen Receptor Gene with Recurrent Spontaneous Abortion (RSA) in Iraqi Women

Authors

  • Shireen Hamid Farhan
  • Ismail A. Abdul-Hassan

DOI:

https://doi.org/10.37506/mlu.v21i2.2837

Keywords:

Androgen receptor gene, gene expression, single nucleotide polymorphism and recurrent spontaneous abortion.

Abstract

The proposal target was determining the gene expression and single nucleotide polymorphism of Androgen
receptor gene (rs6152 G>T) in blood sample of recurrent spontaneous abortion (RSA) Iraqi females. Fifty
females (n=50) with RSA consulted Al-Elwiya teaching hospital from February to June 2019 and apparently
healthy fertile control (n=50) were enrolled. DNA and RNA were extracted from leucocytes to detriment
the SNP and gene expression which were carried out by (RT-q PCR). The results showed non -significant
difference (p>0.05) in the mean of threshold cycle(?Ct) of androgen receptor gene between female with RSA
and control. Assessing the 2???Ct in female with RSA, non -significant decreased expression of folding in
androgen receptor m RNA compared with control. The genotypes and allele frequencies in the two groups
showed significant decrease in the heterozygous (GA) and mutant (AA) genotype frequencies in female with
RSA, the frequency of wild (GG) genotype is increased in female with RSA. In conclusion, expression of
androgen receptor gene didn’t affect in female with RSA, the mutant genotype may play as protective SNP
against RSA.

Author Biographies

Shireen Hamid Farhan

Scholar Researcher, Department of Pathological Analysis, Applied Science, University of Fallujah, Iraq

Ismail A. Abdul-Hassan

Scholar
Researcher, Institute of Genetic engineering and Biotechnology for postgraduate studies, University of Baghdad,
Iraq

Published

2021-03-12

How to Cite

Shireen Hamid Farhan, & Ismail A. Abdul-Hassan. (2021). The Association of Gene Expression and Single Nucleotide Polymorphism (rs 6152 SNP) in Androgen Receptor Gene with Recurrent Spontaneous Abortion (RSA) in Iraqi Women. Medico Legal Update, 21(2), 1126-1132. https://doi.org/10.37506/mlu.v21i2.2837