Efficacy of Acellular-Lyophilized Human Umbilical Cord Powder Seeded with Human Umbilical Cord Mesenchymal Stem Cells in Peripheral Nerve Regeneration in Dogs Models

Authors

  • Ali A.N. AL-Zaidi
  • Zeyad A. Shabeeb
  • Hameed A.K. Al-Timmemi

DOI:

https://doi.org/10.37506/mlu.v21i2.2876

Keywords:

Umbilical Cord, Decellularization, Mesenchymal Stem Cells, Peripheral Nerves, Dogs

Abstract

Treatment of injuries to peripheral nerves after a segmental defect is one of the most challenging surgical
problems. Therapies for peripheral nerves injury are largely ineffective. Therefore, the current study was
conducted to investigate effects either acellular human umbilical cord tissue powder on repair 1 cm critical
size defect radial nerve guided by bovine urinary bladder matrix conduit alone or seeded with mesenchymal
stem cells derived from human umbilical cord. For this purpose, sixteen healthy mongrel dogs were used.
They were randomly divided into two equal groups (n=8), before proceeding with the surgical procedures,
the dogs were generally anesthetized and aseptic conditions were established. Then, the surgery was carried
out with the aid of magnifying lens. All dogs subjected to radial nerve neurotmesis from the right limb. In
the scaffold group, a 1cm of radial nerve was transected and resulted gap bridged with a portion of 14mm
acellular bovine urinary bladder submucosa conduit which was then filled with 0.05mg acellular human
umbilical cord powder and then sutured to the two stumps using 6-0 nylon epineural by simple interrupted
pattern. In combination group, the gap was bridged with acellular bovine urinary bladder submucosa conduit
which was filled with 0.05mg acellular human umbilical cord powder then seeded with 50 µl (5x106)
mesenchymal stem cells derived from human umbilical cord, then fixed onto two stumps using 6-0 nylon
epineural by simple interrupted pattern. Each group was further divided into two equal subgroups (n=4)
categorized according to the post operation periods, that were 8 and 16th weeks for neurohistopathological
examinations. The histopathological examination of regenerated nerve sections (two samples of 5 mm length
each) were collected from the middle (coaptation site) and distal segments were used to determine the degree
of the radial nerve regeneration. The histopathological findings relative convergence between the scaffold
and combination groups in their components such as the increased number of Schwann cells proliferation,
parallel arrangement of nerve fibers and remarkable angiogenesis on 16th weeks. In conclusion; this study
showed that the decellularized both scaffolds alone or seeded with mesenchymal stem cells derived from
human umbilical cord could support the radial nerve regeneration and allow the reinnervation of the target
organ.

Author Biographies

Ali A.N. AL-Zaidi

Lecturer, Department of Clinics Sciences, College of Veterinary Medicine, University of Kufa, Iraq

Zeyad A. Shabeeb

Assistant
Professor Dr., The National Center of Hematology, Al-Mustansiriyah University, Iraq

Hameed A.K. Al-Timmemi

Professor, Departments of
Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad

Published

2021-03-12

How to Cite

Ali A.N. AL-Zaidi, Zeyad A. Shabeeb, & Hameed A.K. Al-Timmemi. (2021). Efficacy of Acellular-Lyophilized Human Umbilical Cord Powder Seeded with Human Umbilical Cord Mesenchymal Stem Cells in Peripheral Nerve Regeneration in Dogs Models. Medico Legal Update, 21(2), 1324-1332. https://doi.org/10.37506/mlu.v21i2.2876