Purification of Gamma Glytamyl Transferase and Study Some Biochemical Variables in Sera Patients with Hepatitis B and C in Kirkuk City

Authors

  • Ibrahim Hadi Salih
  • Sayran S. Saleh

DOI:

https://doi.org/10.37506/mlu.v21i2.2886

Keywords:

Gamma GlytamylTransferase; HCV; HBV; Kirkuk; Liver enzymes.

Abstract

Hepatitis B and C are the most frequent causes of chronic hepatitis diseases in the world. A cross sectional
study was carried out in Kirkuk city from 17th of November 2019 to 25th of February 2020 . The number of
hepatitis patients under study was 80 hepatitis patients, 44 with hepatitis B (14 acute and 30 chronic) and 36
with hepatitis C (11 acute and 25 chronic) Their ages were between 20-70 years old. These patients admitted
to Hepatology and Gastroenterology Center of Azadi Teaching Hospital and Kirkuk General Hospital and
The control group matched the patients group included 30 individuals who were apparently healthy who were
conducted in this study to determine the level of activity of the enzyme (GGT) as well as to determine the
level of a number of biochemical variables (ALT, AST, ALP, TSB, CRP, AFP) in both healthy subjects and
patient group with acute and chronic viral hepatitis B and C . The results showed that there was significant
increase in the activity of the GGT enzyme in patients with P-value (p? 0.01) compared to control group. The
present study showed that there was significant increase in the levels of (ALT, AST.ALP, TSB) in the blood
serums of patients with acute hepatitis B and C compared to chronic and the healthy group. The present study
also included the purification and partial isolation of the enzyme (GGT) and find the approximate molecular
weight for it, that was purified from the blood serum of patients with hepatitis B and C and control group by
using ammonium sulfate, Dialysis and gel filtrationchromatography with sephadex (G-150) to separate the
enzyme from other proteins. The degree of purification in type C was (31.2) times, type B (27) times, and
with healthy (30.2) times. The highsingle peak were used to determine the approximate molecular weight of
the enzyme, and using the gel filtration technique. The approximate molecular weight of the enzyme (GGT)
was up to (371,000-346,000-316,000) Dalton for healthy and hepatitis C and B patients, respectively. In
acute and chronic viral hepatitis B and C, there was a positive correlation between the efficacy of (GGT) and
the efficacy of GPT, CRP and AFP. The study showed that there was significant increase in the activity of the
GGT enzyme in patients compared to control group. The results showed in Table 3 that there was significant
increase in the activity of the ALT enzyme in patients compared to control group. After performing the
process of separating and purifying the GGT enzyme from the serum of patients with viral hepatitis B and
C, using 40-60% of ammonium sulfate and after obtaining the required purity separating it from the rest of
the components of the blood serum, the activity of the GGT enzyme was measured and it was found to have
overall effectiveness in patients with hepatitis Liver C was (0.282) and the number of purification times
was (1.63) times and the specific effectiveness was (1843). Hepatitis B was (3.5) times, and the results from
the dialysis process of the precipitate resulting from the saline precipitation process showed an increase in
the specific activity of the enzyme GGT, so it was (3.4) for healthy people, (3.521) for patients with HCV
and (4.3) HBsAg. Then he used gel filtration chromatography using the gel (Sephadex G-150), the results
showed that a single protein beam appeared, which is highly effective and has a degree of purification
reaching (30.2) And (31.2) and (27) times for GGT enzyme separated by gel filtration technique when the
protein solution obtained from the blood serum of healthy subjects and patients with viral hepatitis is passed
from the separation column containing gel (G-150).

Author Biographies

Ibrahim Hadi Salih

M. Sc. Chemistry Student. College of Science, Kirkuk University, Iraq

Sayran S. Saleh

Prof. Dr. Biochemistry. College of Science, Kirkuk University, Iraq

Published

2021-03-12

How to Cite

Ibrahim Hadi Salih, & Sayran S. Saleh. (2021). Purification of Gamma Glytamyl Transferase and Study Some Biochemical Variables in Sera Patients with Hepatitis B and C in Kirkuk City. Medico Legal Update, 21(2), 1388-1396. https://doi.org/10.37506/mlu.v21i2.2886