One-Pot Synthesis of New Pyrazolo [3, 4, -d] Pyrimidine Derivatives and Study of their Antioxidant and Anticancer Activities
DOI:
https://doi.org/10.37506/mlu.v20i2.1124Keywords:
Pyrazole, Pyrimidine, anticancer, antioxidant DPPH; MCF-7.Abstract
An effort was made to synthesize a new series of pyrazolo [3,4-d] pyrimidine derivatives (2-5). The synthesis
of these compounds was achieved via reaction of 3-methyl-1,4-dihydropyrazol-5-one (1) with a substituted
aromatic aldehyde and urea or thiourea in mild reaction conditions, giving satisfactory yields. FT-IR and
1H-NMR spectroscopies were used to characterize the structure of the newly synthesized compounds (2-
5), where the spectral data confirmed the formation of these compounds. The antioxidant activity of these
compounds (2-5) was examined in this study the synthesized compounds showed a moderate-high antioxidant
effect, which was examined by using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assays.
The cellular toxicity of the compounds (2-5) was studied on MCF-7 cell lines using MTT assay. Compound
(5) showed the highest toxicitytowards MCF-7 cell lines.
