Genotyping of Echinococcusgranulosus Isolates from Human, Sheep and Cattles Hydatid Cysts in Some Central Euphrates Provinces, Iraq.

Abstract

A molecular study was conducted for the period from August 2018 till March 2019. This study included the
collection of 60 samples of human and animal hydatid cysts (sheep and Cattles), human samples collected
from Al-Diwaniyah hospital. Whereas the animal samples were collected from Al- Najaf and Al-Diwaniyah
provinces abattoirs .DNA was extracted from the germinal layer of cysts (for humans, sheep and cows) as
well as from the protoscolices of fertile cystic fluid. The PCR series reaction technique was performed using
touch down for the multiplication of the Cox1 gene. Molecular examination showed that DNA extracted from
protoscolices was better than DNA extracted from the germinal layer when PCR genes were amplified. The
gene sequencer method and the phylogenetic tree analysis were conducted for nine PCR samples consisting
of three samples for everyone humans, cows and sheep. The results showed that there are three strains in
Iraq: sheep strain (G1), cattle strain (G3) and camel strain (G6). The current study reveals the cattle strain
(G3) It was the most present and distributed by 66.6% and this proves that they are most closely related
associated with human, sheep and cattle infections.