Detection for Virulence Factors of Amoebic Dysentery in Bloody Diarrheal Children Under 7 Years

Abstract

Background: Amoebiasis, or amoebic dysentery, is a term used to describe an infection caused by the
protozoan Entamoebahistolytica.
Aim: Identify E.histolytica virulence factors (amoabapore and cysteine proteinase) that play a critical role
in pathogenesis ofamoebic dysentery by using PCR results.
Method: Detect the major virulence factors of the intestinal parasite E.histolytica on Stool samples were
collected from 56 samples by using PCR techniqueand. The DNA sequencing analysis was performed for
confirmative genetic identification of some local Entamoebahistolytica.
Result: To detect the major virulence factors (V.F.) (cysteine proteinase and amoebapore) of E.histolytica,
PCR technique was conducted, by using specific primers for E.histolytica, a 56 samples were positive to
E.histolytica using PCR technique was diagnosed previously,the result showed that 54 stool samples were
bloody & positive to virulence factorcysteine proteinase, and 37 stool samples were bloody & 53 samples
were positive to virulence factor Amoeba pore.
Conclusion: Cysteine proteinase and Amoebaporesis the most important virulence factors in E.histolytica
that play a critical role in the mediated intestinal cell lysis.