Antibacterial Activity of Selected Fractions Extract of Coptis chinensis Franch Rhizomes
Keywords:Coptis chinensis, Fractions, Antibacterial activity, Zone of inhibition
The antibacterial activity of different fractions of selected extract of Coptis chinensis Franch rhizome
was evaluated on gram positive bacteria. Three extracts were tested at concentrations ranging from 1 mg/
ml, 10 mg/ml, and 100 mg/ml in vitro on three bacterial strains, Staphylococcus aureus, Streptococcus
pneumoniae, and Bacillus anthracis, which was done by performing disc diffusion for growth inhibition. The
methanol extract at concentrations of 100 mg/ml displayed the most significant zone of inhibition of (22.67
± 3.055mm), (21.67 ± 3.512 mm), (16.67 ± 1.528 mm) against Streptococcus pneumoniae, Staphylococcus
aureus, and Bacillus anthracis, respectively. The TLC separation of the extracts showed that each of them may
contains constituents that are phenolic in nature. From the Rf values of the spots found in the various extracts
is that the Coptis chinensis Franch rhizome contains some other non-phenolic compounds responsible for its
effect. The selected methanol extract is then subjected to column chromatography and collected 4 fractions.
Out of the four fractions, F2 fraction demonstrates the most significant zone of inhibition of 15.00 ± 1.000
mm, 12.33 ± 1.528 mm and 10.00 ± 2.000 mm against Streptococcus pneumoniae, Staphylococcus aureus,
and Bacillus anthracis, respectively.