Viability Test of Collagen Extract from Gouramy Scale (Oshpronemusgouramy) on Bone Marrow Mesenchymal Stem Cell Culture

Authors

  • Chiquita Prahasanti1, Widya Rizqi Romadhona2, Shafira Kurnia S.2, Aristika Dinaryanti3

DOI:

https://doi.org/10.37506/mlu.v20i4.1786

Keywords:

Bone marrow mesenchymal stem cell, gouramy scales collagen, viability.

Abstract

Background: The role of type I collagen is as a matrix of extracellular proteins with characteristics of
increased cell proliferation which directly affects the physiology and morphology of cells. Type 1 collagen
can be obtained from fish scales. Thus, this research aimed to support engineering tissue used for the
treatment of periodontal disease in the regenerative surgery by utilizing collagen derived from gouramy
scales. As an initial step, the researchers wanted to conduct a study used collagen extract derived from
gouramy scales (Osphoronemusgouramy) which was applied to bone marrow mesenchymal stem cell
cultures to see viability in vitro.
Objective: To determine the viability of collagen from fish scales (Osphronemusgouramy) to bone marrow
mesenchymal stem cells. Method: Bone marrow mesenchymal stem cells are taken from mice and planted
in 96 well plates. Collagen extracted from gouramy scales using the enzymatic method was dissolved in a
condition medium with each concentration of 0.01 mg/ml, 0.02 mg/ml, 0.04 mg/ml, 0.16 mg/ml, 0.32 mg/
ml was put into the well prepared and incubated for 24 hours for the MTT assay.
Result: Collagen from fish scales can increase the viability of bone marrow mesenchymal stem cells with
a percentage above 90% and the highest viability concentration at 0,01 mg/ml. Conclusion: Collagen from
fish scales is viable against bone marrow mesenchymal stem cells. Collagen scales of gouramy soaked in
medium had the highest viability with an optimum dose of 0.01 mg/ml.

Author Biography

  • Chiquita Prahasanti1, Widya Rizqi Romadhona2, Shafira Kurnia S.2, Aristika Dinaryanti3

    1Professor in Department of Periodontics Dental Medicine, Faculty of Dental Medicine, Universitas Airlangga,
    Surabaya Indonesia, 2Dental Medicine Professional Program, Faculty of Dental Medicine, Universitas Airlangga,
    Surabaya Indonesia, 3Research Center for Stem Cell Research and Development Institute of Tropical Disease,
    Universitas Airlangga, Surabaya Indonesia

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Published

2020-11-18

How to Cite

Viability Test of Collagen Extract from Gouramy Scale (Oshpronemusgouramy) on Bone Marrow Mesenchymal Stem Cell Culture. (2020). Medico Legal Update, 20(4), 149-153. https://doi.org/10.37506/mlu.v20i4.1786

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