Biofilm Bacteria and Cholesteatoma on Chronic Suppurative Otitis Media
DOI:
https://doi.org/10.37506/mlu.v20i4.1863Keywords:
Chronic Suppurative Otitis Media, Cholesteatoma, Bacterial Biofilm.Abstract
Background: Chronic suppurative otitis media (CSOM) possibility to cause morbidity and mortality. Germ
infections were a major factor in the long-standing inflammation of CSOM. Cholesteatoma is an epithelial
cyst that containing keratin desquamation. The cholesteatoma present in CSOM patients is a kind of acquisita
with several theories that have been believed to be the basis of its formation.
Objective: To explain the correlation between bacterial biofilms and cholesteatoma in CSOM.
Method: We performed the prospective collection of tissue during mastoidectomy surgery from CSOM
patient. The tissue samples divided into two groups, cholesteatoma and non-cholesteatoma. All tissues
group processed for scanning electron microscopy (SEM) analysis. The SEM results will be categorized
into positive and negative bacterial biofilms.
Result: Statistical analysis using logistic regression test had p = 0.027 and risk ratio = 5.55. That means
there was a significant correlation between bacterial biofilm and cholesteatoma in CSOM patients (p <0.05).
CSOM with cholesteatoma has obtained the risk of bacterial biofilm by 5.55 times compared to CSOM
without cholesteatoma.
Conclusion: There was a correlation between bacterial biofilms and cholesteatoma in CSOM patients.
A quarter of cholesteatoma showed positive biofilms. CSOM with cholesteatoma has a risk of positive
bacterial biofilm.