Association of LPL Gene Variant and Serum LPL Level with Ischemic Stroke in Iraqi Population

Abstract

Background: Lipoprotein lipase (LPL) plays an important role in plasma lipoprotein metabolism.
An increasing number of studies have suggested an association of LPL gene variants with the risk of
cardiovascular and cerebrovascular diseases.
Objective: To analyze whether Hind III polymorphism of LPL gene and serum LPL level are associated
with ischemic stroke in Iraqi population.
Method: Fifty ischemic stroke patients (clinical diagnosis and x-ray CT) and fifty controls were enrolled in
this case–control study. The LPL Hind III polymorphism was determined by PCR-RFLP technique and LPL
mass level was estimated using a sensitive sandwich enzyme-linked immunosorbent assay (ELISA).
Results: In the present research was not found any association between the Hindlll LPL gene polymorphism
and acute ischemic stroke in the population studied; the allele and genotypic frequencies of the studied
polymorphism was similar in cases and controls and followed the Hardy-Weinberg equilibrium. The
relationship between Hind III genotypes and the LPL mass level was analyzed using ANOVA and further
confirmed by Post-hoc analysis. there was no significant difference in LPL mass levels between the genotype
groups H+H+, H+H-, and H-H- (p value>0.05).
Conclusions: The Hind III polymorphism of LPLis not a genetic marker for the development of ischemic
stroke as well as not determinants of serum LPL level in the Iraqian sample used.