Correlation of Glycated Haemoglobin with Netrin-1 and High Sensitive C-Reactive Proteinin Type 2 Diabetes Melitus Patients

Abstract

Diabetes mellitus is a group of metabolic disease with the characteristic of hyperglycemia that occurs due
to abnormal insulin secretion, insulin action or both. Low level inflammation plays a role in the mechanism
of type 2 DM. Netrin-1 is a laminin protein group that has anti inflammatory properties. Netrin-1 is a new
potential biomarker for diagnosing type 2 DM and its complications. High sensitive C-reactive protein is an
acute phase protein produced by the liver in response to proinflammatory cytokines. Glycatedhaemoglobin
test is a test recommended by the American Diabetes Association as a marker for diagnosis and predictor of
the development of DM complications. The purpose of this study was to determine the correlation between
glycatedhaemoglobin with netrin-1 and high sensitive C-reactive proteinin patients with type 2 DM.
This cross sectional studyused type 2 DM patients as a sample. Fifty two type 2 DM patients participated in
this study. The sample consisted of 21 samples of controlled type 2 DM and 31 samples of uncontrolled type
2 DM. The glycatedhaemoglobin was measured using immunoturbidimetry method, netrin-1 was measured
using the ELISA method and hsCRP by immunoturbidimetry method . Data were analyzed statistically with
the Mann Whitney and Spearman test.
The results showed that netrin-1 in the uncontrolled type 2 DM group was higher than netrin-1 in the
controlled type 2 DM group but there was not statistically significant (p> 0.05). High sensitive C-reactive
protein in the uncontrolled type 2 DM group was higher than in the controlled type 2 DM group (p< 0.05).
Glycatedhaemoglobin does not correlate with netrin-1 (p>0.05). Glycatedhaemoglobin was positively
correlated with hsCRP with moderate correlation strength (p < 0.01, r = 0.418). Netrin-1 did not correlate
with hsCRP (p > 0.05). It was concluded that netrin-1 and hsCRP were higher in uncontrolled DM. The
higher glycatedhaemoglobin, the higher hsCRP too.