The Effects of Model IVS 2 MAP Progesterone, eCG, GnRH, and PGf2? on Pregnancy of Tail Fat Ewes Strain Sapudi Islands Subjected to A Short-term Synchronization of Estrus

Abstract

The objective of this study was to verify the effects of engineering design of IVS 2 progesterone with 2
cm thick sponge and 2 cm length and 5 cm silicon plate in the middle with 13 cm nylon cord ties to pull
the sponge position. Try to determine the effective dose of progesterone (MAP) with a dose of 60 mg
progesterone, eCG, GnRH and PGf2? on pregnancy of ewes subjected to a short-term synchronization of
estrus. Tail fat sheep ewes strain sapudi Islands received 60 mg MAP sponges model IVS 2 during 12 days
plus 500 IU eCG folligon and 2.5 mg d-cloprostenol capriglandin (0.5 ml) submucosa vulva 24 h prior to
IVS 2. Control groups (T0) 10 Ewes were assigned to receive 0.9% NaCl solution 2 ml im only when estrus
sign showed waited for natural estrus. Group treatment 10 Ewes were (T1) IVS2 + 500 IU eCG folligon and
group treatment 10 Ewes were (T2) IVS2 +500 IU eCG folligon 21.5 µg (0.5 ml) GnRH fertagyl gonadorelin,
24 hours after withdrawal. Each group was assigned to intracervical insemination by Vaginoscopy (n = 30).
Artificial insemination was performed with a single dose of fresh semen on egg yolk skim milk dilluters.
For controlled mating, females were exposed to males 48 hours after sponges removed. Ten females per
treatment were subjected to transrectal ultrasound examination at 35 days late after AI. Estrous response
(100.0%), pregnancy rates (T0 80% T1 70% and T2 70%), did not differ between T1 and T2 respectively (p
>0.05). Administration of GnRH 24 hours after IVS 2 removed does not improve pregnancy rate in estrous
synchronization in Tail fat ewes strain sapudi Islands (p >0.05) on day 35.