New Media from Plant Extracts for Isolation of Some Pathogenic Bacteria that Produce Protease on Linum Usitatissium Seed Powder Agar and to Identify Serratia Marcescens
DOI:
https://doi.org/10.37506/mlu.v20i4.2072Keywords:
Prodigiosin, Trigonella foenum-graecum, Serratia marcescens, pigment.Abstract
Prepared two concentration of four new enriched media for isolation of some pathogenic bacteria
including Acinetobacter baumannii, Klebsiella pneumoniae, Escherichia coli, and Serratia marcescens and
identification of Serratiamarcescens that produce red pigment (prodigiosin) . The first concentration 12.5 g/l
of plant extracte showed heavy growth of pathogenic bacteria on the four media . The second concentration
25 g/l showed also growth of the pathogenic bacteria incubation in 37°C for 24h except on the Elettaria
cardemomum agar and, Fenugreek (Trigonella foenum-graecum) seeds agar. Serratia marcescens produce
red pigment on all the new media in both concentration . All isolates produce protease enzyme on Linum
usitatissium seed agar . The result is a synthesis enrichment and inexpensive medium for different species
of bacteria like Linumusitatissium enriched with a different materials and salt as amineral that suitable for
growth organism without any morphological change of metabolisms feature, also identification Serratia
marcescens by produce red pigment that important in medical with a wide range of biological activities.