Assessment of the Two-Glutathione S-Transferase Genotypes GSTM1 and GSTT1 Polymorphism Frequencies in Epithelial Ovarian Cancer in Nasiriya Province

Abstract

The family of glutathione S-transferases (GSTs) is composed of multiple isozymes with significant evidence
of functional polymorphic variation. Glutathione S-transferase GSTM1 and GSTT1 are members of the
GST family, is responsible for metabolism of xenobiotics and carcinogens. Allelic variant of GST gene
polymorphisms might impair detoxification function and increase the susceptibility to cancer. The null
genetic polymorphism in the gene encoding GST Class µ, GSTM1 and GSTT1, has been reported to be
significantly elevated in cancer patient. This study aimed to investigate if these polymorphisms are useful
markers for predicting ovarian cancer susceptibility. 30 cases of epithelial ovarian cancer and 30 controls
was used to investigate the frequency of GSTT1 and GSTM1 genotypes by using multiplex conventional
PCR protocol. The frequency of GSTM1 null allele was significantly elevated (100%, P = 0.004) compared
with GSTT1 active allele (13% P = 0.037). GSTT1 null showed no significant effect in prognosis epithelial
ovarian cancer (17%, P = 0.551). We conclude that the GSTs might affect in both risk to ovarian cancer.