Detection of Copy Number of Mitochondrial DNA in Iraq Population

Abstract

Mitochondrial DNA copy number (mtDNA-CN), a measure of the number of mitochondrial genomes per
cell. A cell contains two copies of genomic DNA compared to a single copy of DNA per chromosome. The
quantitative real-time PCR (qPCR) is the gold standard method for measuring mtDNA-CN, the quantification
after DNA extraction it is an important step, the quantitation of DNA plays a central role inmedical and
forensic DNA analysis, which provides the information about the amount of DNA present in unknown
samples. In this study the quantitative results showed that the higher value 74941 and the lower value 190.
A multiplex quantitative PCR assay developed to amplify target sequences of different length, which allows
for the assessment of DNA degradation in samples of forensic interest. The quantification of DNA by qPCR
relies on the detection of amplified product (amplicon) at each cycle of the PCR.