Vitik Detection of Aerobic Spore-Forming Bacteria Isolated from Raw Milk, Skim Milk Powder and UHT Milk

Abstract

Background Aerobic spore-forming bacteria can be found in a wide range of environmental niches such
as food production. Among them, the aerobic spore-forming bacteria like Bacillus ceruse, pose a risk of
causing dairy product poisoning by the production of toxins.
Materials and Method: Out of 40 samples, 10 from each of Raw Milk, Skim Milk Powder, UHT Milk and
Wight chees were collected to isolate aerobic spore-forming bacteria by morphological, physiological and
biochemical tests.
Conclusion: A total of 25 isolates of heat resistant bacteria, 10(40%), 8(32%), 3(12%), 4(16%) from “RM,
white chees, SMP and UHT milk respectively,” were purified and characterized. Various spore forming
bacteria belonging to Bacilli spp. which include pathogenic were biochemically identified using vitek 2
systems, the result showed the highest rate of Bacillus subtilis (28%) followed by Bacillus ceruse and
Bacillus thuringiensis (24%).