Evaluating the Sensitivity of (miR-378) as a Circulatory Screening Biomarker for Diabetic Cardiomyopathy: Comparative Study

Abstract

Background: Diabetic cardiomyopathy is one of the serious complication of diabetes mellitus, with a silent
development and it is often underestimated, Currently available diagnostic techniques are limited in their
ability to identify patients who present with uncommon symptoms or silent myocardiopathy in its early
stages.
Aim: To evaluate role of miRNA-378 compared to GLS as a sensitive biomarker for early detection of
subclinical diabetic cardiomyopathy.
Method: This case control study involved 75 DM2 patients from Merjan Medical City in addition to age
and sex-matched 25 apparently healthy subjects . The echocardiographic assessment for GLS was done
for control group (H) and normotensive diabetic patients and these patients were divided into (CD) group
without cardiomyopathy, and (M)group with cardiomyopathy diagnosed by negative GLS and positive GLS,
respectively. Group (F) include Hypertensive and/or ischemic diabetic patients with overt heart failure .
conventional Echocardiography was performed to all patients and control groups, all of them were of normal
EF, except group F who have EF less than 40%. Bloodsampling was done for all and miRNA extraction and
analysis done according to the manufacturer recommendations.
Results: There is significant elevation in miR-378 Ct value between H in one side & other three diabetic
gps. (CD, M, F) as presented in table (3-17) (p< 0.05). There is significant elevation in miR-378 Ct value in
M than CD groups. There is significant elevation in Ct value in F than M gp. (p=0.000). In the ROC curve
(AUC) was 0.965. The sensitivity and specificity were 0.978 and 0.646, respectively according to the chosen
cut-off value.
Conclusion: This study showed that miRNA-378 could be regarded as a novel sensitive biomarker which
could play a significant role for early detection of subclinical diabetic cardiomyopathy