Human Folate Receptor-1 Gene Expression and Correlation with Physiological Leptin hormone in Obese and Non-obese Subjects

Abstract

Background: One of the most important agents that must be found in all dividing cells is folate.
Aim: Assessment of human folate receptor-1 gene expression and correlation with leptin hormone in obese
and non-obese subjectsof Babylon province, Iraq.
Subjects and Method: This study included 60 obese subjects (OS) in the range of age (10-45 years) and 30
normal weight subjects (NOS) in range age of (11-43 years). FOR-1 gene expression were assessing by qRTPCR after RNA extraction from fresh blood of all obese and normal subjects and immediately converting
into cDNA. Leptin was assessed by ELISA method.
Results: The results suggesting that founds the positive correlation (r2
=0.447) between ages of OS and
levels of LEP (ng/ml). The results of this study showed significant differences (p-value< 0.05) in levels of
leptin (LEP) between OS and NOS groups. The results showing that the FOR-1 gene expression ratio in
NOS compare to expression OS were 11.9 and 7.1.
Conclusion: FOR-1 have a higher expression in OS compare to NOS and this may be useful in diagnostic
and therapeutic tools in diseases or cancers in OS.