Evaluation of the Antioxidant Activity of the Polyherbal (Conocarpus lancifolius L., Capparis spinosa L. and Dodonaea viscosa) Extracts and Assessment of the Hypoglycaemia Effect in Diabetic Mice
Keywords:polyherbal, total phenol, antioxidant, hypoglycaemia, diabetic mice.
Diabetes mellitus, often referred to simply as diabetes, is a chronic metabolic disorder due to the relative
deficiency of insulin secretion and varying degrees of insulin resistance. It is characterised by high circulating
glucose. Excessive levels of either molecular oxygen or Reactive Oxygen Species (ROS) lead to an imbalance
in the body’s normal oxidative metabolism that to leads to high glucose levels in the blood (hyperglycaemia),
resulting in metabolic disturbances (oxidative stress) and chronic complications in diabetes. The present
study aims to estimate the antioxidant activity in diabetic mice induced via alloxan of a combination of
three types of plant leaves (Conocarpus lancifolius L., Capparis spinosa L. and Dodonaea viscosa). The
total phenolic content using Folin-Ciocalteu reagent and the antioxidant activity utilizing 2, 2-diphenyl-
1-picrylhydrazyl (DPPH) assay were estimated. The effect of polyherbal formulation leaves extracts on
serum glucose level was done on forty-two albino mice divided into six groups and treated with polyherbal
extracts and metformin. The results of total phenolic content in the polyherbal leaves extracts was observed
(15.52 mg\g) in the aqueous extracted samples, while the total phenolics content was (46.97mg\g) in the
methanolic extract, and the antioxidant activity showed the methanolic extract was the highest free radical
scavenging activity (93.28%) than the aqueous extract (86.77%) in 10 mg/ml and approach to the artificial
antioxidant Butylated hydroxytoluene (BHT) which was (93.67%). This study showed both extracts of
polyherbal leaves did not induce lethality in mice when administered orally at a dose of 2000 mg/kg. on the
other hand, Diabetic mice treated with methanolic extract at doses 200 and 400 mg/kg showed a significant
decrease (p<0.01) in serum glucose level after 35 days, which was 113.66 and 107.66 mg/dl respectively,
and the aqueous extract was 124.66 and 117.00 mg/dl respectively when compared with the control positive
group (324.00 mg/dl).
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.