TLC Identification of Bacteriocin from Different LAB Clinical Isolates of Najaf Hospitals and in Vitro Evaluation of Its Effectiveness Against three Pathogenic Bacterial Isolates
Keywords:Lactic acid bacteria LAB, bacteriocin, TLC.
Probiotics are useful microorganisms that are effective in protecting against pathogenic microorganisms,
used to support food to provide beneficial effects to human health by maintaining the natural balance of the
intestinal flora and reducing diseases, especially those related to the gastrointestinal tract.The ability of the
Lactic acid bacteria to produce bacteriocin. In this study 74 samples were collected from various clinical
sources include 31 samples of mouth and 43 samples from Vaginal swabs, for the period July 2018 until
December 2018. The results of the isolation and laboratory diagnosis and biochemical testes the ownership
of 43 isolates from lactic acid bacteria in vaginal swab and the highest percentage isolates bacterial (52%)
of the samples of the vagina. All isolates showed lactic acid bacteria effectiveness of the microbial agents
toward some negative bacterial species to dye grams diameters ranged between inhibition zones (14 –
22mm). Findings showed that RF bacteriocin values produced by the bacterium LAB isolates ranged from
(0.45 – 0.57).
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