Eccentric Exercise Decrease Blood Glucose Level and Improve Protein Level of Glucose Transporters in Diabetic Mice Muscle

Abstract

Background: Previously, eccentric exercise improved on blood glucose level better than concentric and
isometric exercise. The improvement of blood glucose level was resulted from higher glucose uptake
activity, particularly at the muscles. Unfortunately here isn’t yet information whether it is facilitated by
glucose transporter type 1 or glucose transporter type 4.
Objectives: The aim of the present study was to investigate the effect of eccentric activity on glucose
transporter type 1 and glucose transporter type 4 in gastrocnemius muscle of streptozotocin-induced diabetes
mellitus mice.
Methods: Diabetic mice were grouped randomly into 4 groups (7 mice each group). Two groups were
observed during fasting and others were observed during post prandial. Single bout of eccentric activity was
given by downhill running on 10o degree decline treadmill. The protein level of glucose transporters were
measured using monoclonal antibody in ELISA protocol. Blood glucose level was measured on fasting and
post prandial using colorimetric protocol of EASY TOUCH app.
Result: Glucose levels (fasting and post prandial) of eccentric group were significantly lower than isometric
group. Consistently, protein level of glucose transporters (type 1 and type 4) were significantly higher than
isometric group. Type-4 glucose transporter level was higher than type-1 glucose transporter, found in calf
muscle.
Conclusions: single bout of eccentric exercise on downhill running improve blood glucose level due to both
GLUT-1 and GLUT-4 protein levels improvement.