Molecular and Classical identification of Staphylococcus aureus, Isolated from Iraqi Patients with Recurrent Tonsillitis

Authors

  • Hawraa Fadhil
  • Bushra Jasim Mohammed

DOI:

https://doi.org/10.37506/mlu.v22i1.3176

Keywords:

Classical diagnosis, Molecular diagnosis, PCR, S. aureus, Recurrent tonsillitis, Essential genes.

Abstract

Tonsillitis is an infection of the tonsils caused by one of several types of bacteria or viruses.
Staphylococcus aureus are one of most common bacteria isolated from recurrent tonsillitis, its
identification by classical methods takes 3-5 days as well as the accuracy does not reach an absolute
degree. So current study aimed to diagnose S. aureus by classical and molecular diagnosis to increase
the accuracy of the diagnosis. Two hundred and fifteen tonsil swabs were collected from Iraqi patients
susceptible suffered from recurrent tonsillitis who attended clinics in major hospitals in Najafe/ Iraq.
Classical diagnoses of S. aureus were done by bacterial cultures, biochemical test and Vitek system.
While molecular diagnosis achieved via PCR technique The results of classical methods showed that
50 isolates recovered from (195) suspected patients were S. aureus, while molecular diagnoses showed
that 45 isolates out of 50 diagnostic S. aureus via classical methods were S. aureus with significant
difference (P≤ 0.01). In conclusion, Molecular method is more sensitive in diagnoses of S. aureus than
conventional testes in case of recurrent tonsillitis.

Author Biographies

Hawraa Fadhil

Scholar Researcher, Institute of genetic engineering and biotechnology, University of Baghdad, Iraq

Bushra Jasim Mohammed

Scholar Researcher, Institute of genetic engineering and biotechnology, University of Baghdad, Iraq

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Published

2021-11-08

How to Cite

Hawraa Fadhil, & Bushra Jasim Mohammed. (2021). Molecular and Classical identification of Staphylococcus aureus, Isolated from Iraqi Patients with Recurrent Tonsillitis. Medico Legal Update, 22(1), 23–30. https://doi.org/10.37506/mlu.v22i1.3176