Cytotoxic Effect of Modified Gutta Percha by Incorporating Bioactive Glass 45S5 and Chitosan Particles As Potential Root Canal Filling Material

Authors

  • Ahmed I AL-Jobory
  • Raghad AL-Hashimi

DOI:

https://doi.org/10.37506/mlu.v22i2.3246

Keywords:

Gutta percha, bioactive bioglass BG45S5, chitosan, MTT assay, fibroblast cells, cytotoxic effect.

Abstract

Aim: To investigate the cytotoxic effects of modified bioactive and antimicrobial gutta percha on
the fibroblast cells.
Methods: The cytotoxic effects of modified bioactive and antimicrobial gutta percha on the
fibroblast cells were investigated using Methylthiazol tetrazolium (MTT) assays at different times
24, 48 and 72 hours.
Results: there is no significant difference between controle and new modified gutta percha at first
24 hrs. while with time at 48 and 72 hrs. there were significant difference.
Conclusion: Bioactive bioglass BG45S5 and chitosan showed in vitro cytotoxic effects when
mixing with gutta percha in fibroblast cells as demonstrated by MTT assays, although toxic effect
was observed mostly as the same to control gutta percha.

Author Biographies

Ahmed I AL-Jobory

Post Graduate, Department of Aesthetic and Restorative Dentistry, College of Dentistry, Tikrit
University, Tikrit, Iraq

Raghad AL-Hashimi

Asist Prof/ Department of Aesthetic and Restorative Dentistry, College of Dentistry, University of Baghdad, Baghdad, Iraq

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Published

2022-04-16

How to Cite

Ahmed I AL-Jobory, & Raghad AL-Hashimi. (2022). Cytotoxic Effect of Modified Gutta Percha by Incorporating Bioactive Glass 45S5 and Chitosan Particles As Potential Root Canal Filling Material. Medico Legal Update, 22(2), 176–184. https://doi.org/10.37506/mlu.v22i2.3246