Cytotoxic Effect of Modified Gutta Percha by Incorporating Bioactive Glass 45S5 and Chitosan Particles As Potential Root Canal Filling Material
Keywords:Gutta percha, bioactive bioglass BG45S5, chitosan, MTT assay, fibroblast cells, cytotoxic effect.
Aim: To investigate the cytotoxic effects of modified bioactive and antimicrobial gutta percha on
the fibroblast cells.
Methods: The cytotoxic effects of modified bioactive and antimicrobial gutta percha on the
fibroblast cells were investigated using Methylthiazol tetrazolium (MTT) assays at different times
24, 48 and 72 hours.
Results: there is no significant difference between controle and new modified gutta percha at first
24 hrs. while with time at 48 and 72 hrs. there were significant difference.
Conclusion: Bioactive bioglass BG45S5 and chitosan showed in vitro cytotoxic effects when
mixing with gutta percha in fibroblast cells as demonstrated by MTT assays, although toxic effect
was observed mostly as the same to control gutta percha.
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