Gross examination and Toxicological Analysis of Gastrointestinal Tract for Dichlorvos Poisoning Caused by Nuvan Insecticide During Post-Mortem Examination: A Case Series
Keywords:Pesticide poisoning; Dichlorvos (DDVP); Forensic Toxicology; Thin layer chromatography (TLC); Nuvan insecticide; Post-Mortem Examination; Clinical Toxicology.
Background: “Nuvan” is a trading name for Dichlorvos (DDVP) organophosphate insecticide. Nuvan contains
76% DDVP and is accessible in India. Nuvan Insecticide is highly misused as orally ingested in suicide attempts
and is one of the leading causes of poisoning deaths in Indian rural areas.
Methods: In this case study, we have diagnosed the four poisoning deaths by Nuvan-dichlorvos insecticide
through Post-Mortem examination and toxicological analysis. The gastro-intestinal tract (GIT) was grossly
examined. The solvent extraction method is used for withdrawing pesticide content from GIT. Toxicological
analyses were performed using thin-layer chromatography (TLC) using a spraying reagent
Results: A Fluorescent turquoise blue liquid content was found throughout the stomach to the small intestine.
Content had a notable odor of volatile organic compounds (VOCs). The mucosal walls of the stomach were highly
congested and hemorrhagic. Toxicological analyses done for all four cases showed a confirmatory detection of
Conclusions: The simple gross examination of gastrointestinal viscera during post-mortem is supporting
diagnostic evidence for orally administered Nuvan insecticide. Solvent extraction method is a suitable technique
for the extraction of pesticides from aqueous biological matrices. Thin Layer Chromatography is a simple and
inexpensive technique for chemical confirmation and DDVP was detected.