Role of MnSOD Val16Ala Gene Polymorphism in Changing the Level of Serum Metals in Workers Exposed to Heavy Metals in Al-Nasiriyah City

Abstract

Low activity of antioxidant enzymes due to polymorphism in antioxidant genes and differences in mineral and metal levels creates oxidative stress that may play a role in advancing many diseases. The aim of this study was to determine the role of Manganese superoxide dismutase (MnSOD) gene polymorphism [rs4880 Val16Ala)] in exposed workers and to compare the levels of metals and minerals among its various genotypes in control group. A total of 80 individuals including age and gender matched workers and control group were genotyped. Detection of rs4880 polymorphism was done using sequencing technique. The blood serum were tested for determination of metals and minerals using Atomic absorption spectrophotometer (AA 6600 Shimadzu). This study indicate that the frequency of T allele was higher than that of C allele in control group as well as in workers. Our results indicate no association between all genotypes in study subjects. The results of present study indicates that Pb and Cd concentration differ significantly between TT genotypes of MnSOD (rs4880) gene polymorphism as compared to CC genotypes in workers and control group (p<0.05). Control group with TT, TC and CC genotypes have high concentration of Fe, Cu and Zn as compared to workers group.